Histone methylation changes are required for life cycle progression in the human parasite Schistosoma mansoni
Fig 2
Sample clustering and genome-wide frequency of combinatorial states during the life cycle of Schistosoma mansoni.
(A) ChIP-seq data heatmap between five parasite developmental stages for H3K4me3 and H3K27me3. Typical example of chromatin profile for H3K4me3 (B) and H3K27me3 (C). (D) Genome-wide frequency of all combinatorial states in all stages. Two waves of repressive H3K27me3 (red line) with a maximum of methylation can be seen in Sp1 and adults, while a constant line of H3K4me3 (green line) is seen throughout the whole cycle (left Y-axis). A single wave of bivalent chromatin (H3K4me3 and H3K27me3 co-localizing, purple line, secondary axis) has its maximum in cercariae (right Y-axis). (E) Frequency methylation at TSS. Intense repressive methylation inside intermediate and definitive hosts (H3K27me3, red line, left Y-axis), intense bivalency state in cercariae (H3K4me3+H3K27me3 at same locus, purple line, secondary axis, right Y-axis) with loss of single active H3K4me3 histone mark (H3K4me3, green line, left Y-axis). X-axis: Five developmental stage of S. mansoni; Y- axis: Genome-wide methylation frequency (%).