Shape-shifting trypanosomes: Flagellar shortening followed by asymmetric division in Trypanosoma congolense from the tsetse proventriculus
Fig 3
(A) Images from Trypanosoma congolense 1/148 YPFR time course showing decrease of flagellar length (visualised by YFP::PFR1 fluorescence) over time; details as Fig 2 legend. Note first appearance of PFR1 depot at T = 10 (arrows). The arrowheads indicate that the PFR does not extend to end of cell body (T = 6 to T = 14); white arrowhead, end of PFR; black arrowhead, end of cell body. Brightfield image merged with the YFP image (YFP::PFR1). Scale bar = 10 μm. (B) Length of flagellum (squares) and percentage of trypanosomes with YFP::PFR1 depot (circles) from T = 0 to T = 14. (C) Diagrammatic representation of morphological changes in trypanosomes from T = 0 to T = 14 compiled from morphometric data (S1 Table, S3 Table). The PFR is indicated by the thick black line, with depot shown as a filled rectangle.