Biphasic zinc compartmentalisation in a human fungal pathogen
Fig 11
Zrc1 is essential for zinc detoxification.
(A) Strains were cultured for 24 h in SD0 medium containing indicated zinc supplementation. Experiment performed at least three times in duplicate for zinc concentrations at 25 μM and above. *** indicates significant difference (P < 0.001) compared to wild type and revertant, ANOVA. (B) Strains were precultured in SD0, challenged with 1 M ZnSO4 for 3 h and viability assessed by measuring CFUs. ** indicates significant difference (P < 0.01) compared to wild type and revertant, ANOVA. Experiment performed three times for wild type and zrc1Δ and twice in duplicate for all three strains.