Biphasic zinc compartmentalisation in a human fungal pathogen
Fig 9
Relationship between zincosomes and vacuole in C. albicans.
(A) Cells were co-stained with zinquin (zincosomes) and FM4-64, which stains the fungal vacuole membrane. Note that zincosomes are not intra-vacuolar. (B) The zinc-specific probe Zinpyr-1 can be used to detect vacuolar zinc in C. albicans. Cells were co-stained with Zinpyr-1 and FM4-64. Note that Zinpyr-1 stains vacuolar zinc in C. albicans (C) Zrc1 is not required for vacuolar zinc import. Cells were loaded with Zinpyr-1, pulsed with 25 μM zinc and Zinpyr-1 fluorescence determined at 0, 30, 60 and 180 minutes post pulse. Experiments performed at least twice.