Biphasic zinc compartmentalisation in a human fungal pathogen
Fig 8
Zincosome formation is Zrc1 dependent.
(A) Zincosome screen. Wild type, ZnT deletion mutants and zrc1Δ+ZRC1 strains were pulsed with 25 μM zinc for 20 minutes and zincosome fluorescence determined by staining with zinquin. Prepulsed cells were also stained as control. Experiment was performed at least twice in duplicates and all data normalised to the post-pulse value of wild type. ANOVA was first performed on initial (pre-normalised data). Asterisks indicate statistical significance compared to wild type and to relevant deletion mutant ** P <0.01. (B) As panel A, except zinquin fluorescence kinetics was determined by flow cytometry. Experiment performed three times. zrc1Δ exhibits significantly reduced zinquin fluorescence compared to wild type and revertant at 20 minutes P < 0.001, ANOVA.