Biphasic zinc compartmentalisation in a human fungal pathogen
Fig 2
Zinc uptake by C. albicans is mediated by Zrt1 and Zrt2.
(A) Indicated strains were cultured in low zinc medium (SD0, pH ~4.7), exposed to 25 μM ZnSO4 and zinc acquisition determined at indicated time points by measuring how much zinc remained in the cell free supernatant. C. albicans wild type acquires all measurable zinc within 60 minute; zrt2Δ does not; complementation restored zinc acquisition to 68%. Experiment performed three times (B) Indicated strains were incubated in RPMI without zinc for 24 h, exposed to 25 μM ZnSO4 and zinc acquisition determined as in panel A. Wild type cells acquire 74% of zinc by three hours; uptake is reduced by approximately 50% in zrt1Δ and zrt2Δ. zrt1Δ/zrt2Δ fails to take up zinc. Experiment performed twice. Data points have been shifted to the right to make them visible amongst strains.