Actin polymerization in the endosomal pathway, but not on the Coxiella-containing vacuole, is essential for pathogen growth
Fig 9
Arp2/3 is required for endocytic trafficking of transferrin in C. burnetii infected cells.
(A-C) Two dpi Vero cells treated with CK-666 or DMSO for 24 hr were incubated with 488-transferrin (488Tfr) for 1 hr, washed with PBS, then fixed and immunostained for CD63 and C. burnetii. CK-666 inhibits trafficking of 488Tfr to CCVs, decreasing the amount of 488Tfr on the CCV and colocalization with CCV CD63 clusters. Graphs represent the means ± SD of ≥ 60 cells from 3 independent experiments. Statistical significance was determined by the Student’s t-test (****P <0.0001). NMII, C. burnetii Nine Mile phase II strain. Scale bar, 5 μm.