Loss of CXCR6 coreceptor usage characterizes pathogenic lentiviruses
Fig 3
The inability of SIVcpz to use CXCR6 for entry is determined by Env, not cpzCXCR6.
293T cells were transfected with expression plasmids containing CD4 and coreceptor. The species origin of the CD4 and coreceptor are indicated below the graph (C, chimpanzee; M, mustached monkey;–, empty vector control). 48 hours post transfection, cells were infected with luciferase reporter pseudotypes carrying the SIVcpz MT145 Env (A) or the SIVmus1085 1–54 Env (B). Entry was quantified 72 hours later by lysing cells and measuring luciferase content by relative light units (RLU). Infections were carried out in triplicate and data represent means ± one standard deviation.