Deletion of transketolase triggers a stringent metabolic response in promastigotes and loss of virulence in amastigotes of Leishmania mexicana
Fig 10
Relative levels of different amino acids measured in WT and Δtkt cells and their growth media.
(A) Selected amino acids in fresh and spent media of WT and Δtkt cultures over four days of cultivation as detected by LC-MS analysis, FM—fresh medium, n = 4. (B) Intracellular levels of amino acids in WT, Δtkt, and Δtkt + TKT cultures detected by LC-MS. Intensity detected in WT is considered reference and the other values indicate relative abundance, n = 4. All the amino acids were identified based on matches with respective standards.