Deletion of transketolase triggers a stringent metabolic response in promastigotes and loss of virulence in amastigotes of Leishmania mexicana
Fig 5
Central carbon flux is decreased in Δtkt.
(A) Glucose consumption by WT and Δtkt cell lines. The graph indicates the concentration of glucose detected in spent media of the respective cultures, starting in Homem medium containing 1 mM glucose, n = 4. (B) Metabolic end products as detected in spent media of WT and Δtkt cultures by LC-MS, all three metabolites were identified based on matches with the respective standards. Growth rates were not significantly different. FM, fresh medium.