Nuclear RNA surveillance complexes silence HIV-1 transcription
Fig 5
RRP6, MTR4, ZFC3H1 and ZCCHC8 associate with the promoter-proximal region of HIV-1 chromatin.
(A) Schematic diagram showing the location of primers used to amplify ChIP samples by Q-PCR. Primers used to amplify promoter-proximal and coding region sequences are indicated on the figure. (B) HeLa-LTR-luc cells were analyzed by ChIP for association of RRP6, MTR4, ZFC3H1 and ZCCHC8 with the promoter-proximal and coding regions of LTR-luc, Results are shown relative to an IgG control. Data represent mean ± SEM obtained from 3 independent experiments (**P < 0.01, *P < 0.05, NS indicates not significant, independent Student’s t test). (C) HeLa-LTR-luc cells were analyzed by RNA-ChIP for association of RRP6, MTR4, ZFC3H1 and ZCCHC8 with the promoter-proximal region of LTR-luc. Data represent mean ± SEM obtained from 3 independent experiments (**P < 0.01, *P < 0.05, NS indicates not significant, independent Student’s t test). (D) HeLa-LTR-luc cells transfected with the indicated siRNAs were analyzed by RNA-ChIP for association of ZFC3H1 and ZCCHC8 with the promoter-proximal region of LTR-luc. Data represent mean ± SEM obtained from 3 independent experiments (**P < 0.01, *P < 0.05, NS indicates not significant, independent Student’s t test).