Evolution of structural diversity of trichothecenes, a family of toxins produced by plant pathogenic and entomopathogenic fungi
Fig 5
Functional analysis of TRI17 in Trichoderma arundinaceum.
(A) Quantitation of harzianum A production in the wild-type strain Ta37, tri17 mutant strain tri17.139, and three tri17.139-derived strains (tri17.C1, tri17.C4 and tri17.C10) that were complemented with a wild-type copy of T. arundinaceum TRI17. (B and C) Total ion chromatograms of culture extracts of (B) wild-type progenitor strain Ta37 and (C) tri17 deletion mutant tri17.139. The trichothecene biosynthetic intermediate trichodermol (4-hydroxy EPT) is indicated at 5.268 min. 4OH indicates trichodermol (4-hydroxy EPT).