Outer membrane vesicles from Neisseria gonorrhoeae target PorB to mitochondria and induce apoptosis
Fig 2
OMVs secreted by N. gonorrhoeae contain distinct proteins.
(A) Total membranes of N. gonorrhoeae were separated by sucrose gradient ultracentrifugation and fractions were probed with anti-PorB and anti-BamA to detect fractions with outer membranes and anti-F1-β with inner membranes. Fractions 10 and 5 were used as outer and inner membrane fractions, respectively, in further analysis. (B) OMVs, outer membrane (OM), inner membrane (IM), total membrane (TM) and whole bacterial lysate (WBL) were probed for two outer membrane proteins (BamA and PorB) and inner membrane protein, F1-β. (C) Colloidal coomassie staining of proteins of N. gonorrhoeae secreted outer membrane vesicles (OMV), outer membrane (OM) and inner membrane (IM). The positions of molecular size markers (kDa) are shown on the left. PorB (36 kDa) is indicated on the right and over represented proteins in OMVs by asterisks.