Lipid droplet formation in Mycobacterium tuberculosis infected macrophages requires IFN-γ/HIF-1α signaling and supports host defense
Fig 5
HIF-1α target gene Hig2 is required for LD maintenance during M. tuberculosis infection.
(A) RNA-seq data for transcript levels of Hig2 in wildtype BMDM either unstimulated [U], IFN-γ activated [G], M. tuberculosis infected [TB], or IFN-γ activated and M. tuberculosis infected [TB/G]. Samples were collected 1 day post-infection in 3 independent experiments. (B-E) IFN-γ activated wildtype and Hig2-/- BMDM were infected with M. tuberculosis 635-Turbo. BODIPY 493/503 staining and confocal microscopy was performed 1 day post-infection (B,D) and 3 days post-infection (C,E). CellProfiler was used to quantify (F) the percentage of BMDM with LDs, (G) the number of LDs per BMDM, and (H) LD size. Each data point is quantified from ~500 BMDM. Figures are representative of three experiments, error bars are standard deviation, *p<0.05, **p<0.01, ***p < .001, ****p<0.0001 by unpaired t-test.