Two closely related Rho GTPases, Cdc42 and RacA, of the en-dophytic fungus Epichloë festucae have contrasting roles for ROS production and symbiotic infection synchronized with the host plant
Fig 2
Specific interactions of Epichloë festucae Rho GTPases, RacA and Cdc42, with components of Nox complex.
(A) Alignment of the deduced amino acid sequences for E. festucae Rho GTPases. Conserved domains among Rho GTPases are boxed. Amino acid substitutions introduced for constitutive active (CA) and negative (CN) form of small GTPases are indicated by arrows. (B) Phylogenetic Analysis of Rho GTPase from E. festucae. The tree was prepared by the neighbor-joining method (Saitou and Nei, 1987). The scale bar corresponds to 10 estimated amino acid substitutions per site. Numbers at the nodes indicate the percentage of 1000 bootstrap replicates that supported each labeled interior branch. Ef; Epichloë festucae, Fg; Fusarium graminearum, Mo; Magnaporthe oryzae, Nc; Neurospora crassa. (C) Yeast two-hybrid assays of the interactions between E. festucae NoxR, BemA and Rho GTPases. Rho GTPases have mutation in C-terminal plasma membrane localization signal. Yeast strain AH109 was transformed with prey and bait vector as indicated and plated on to SD medium lacking leucine and tryptophan (-L/-T) or lacking leucine, tryptophan, histidine and adenine (-L/-T/-H/-A). Growth on the latter indicates an interaction between bait and prey. (D) A Model for interactions of Cdc42, RacA with components of Nox complex.