IFNγ inhibits G-CSF induced neutrophil expansion and invasion of the CNS to prevent viral encephalitis
Fig 5
GKO Neutrophils have a GDSC phenotype and suppress T cell proliferation.
(A-C), Spleen cells isolated at day 6 pi from HSV infected WT or GKO mice were labeled with CFSE and stimulated with HK-HSV to determine effector (e) T cell proliferation at indicated times post culture. GKO or WT T cells that have divided at least one time are depicted as line plots as % proliferating (A) eCD4, (B) eCD8 T or (C) H-2Kb HSV-1 specific gB498 e-tetramer+ (e-Tet+) CD8 T cells. Linear regression analysis showed significant deviation of slope for GKO CD4 (P = 0.041), GKO CD8 (P = 0.0232) and GKO gB498 tet+ CD8 (P = 0.0171) but not WT T cells. (D-E) GKO Innate cells including PMN (CD11b+ Ly6G+) or M/DC (Ly6G- CD11b+ / CD11c+ cells) isolated from blood of GKO mice at day 6 pi cultured with CFSE labeled GKO or WT T cells and CD19+ B cells isolated from spleen cells as in A-C were assessed for suppression of proliferation of (D) eCD4 and eCD8 T cells and (E) memory (m) CD4 and mCD8 T cells and m-H-2Kb HSV-1 gB498-505 tetramer+ (m-Tet+) CD8 T cells. Effector (e) T cells were obtained from spleens of HSV infected WT or GKO mice at day 6 pi while memory (m) T cells were obtained from spleens of immunized mice at day 21 pi. Data is combined from 2 experiments, n = 6 mice per group. PMN: Neutrophils, M/DC: Monocytes/Dendritic cells.