eCD4-Ig promotes ADCC activity of sera from HIV-1-infected patients
Fig 2
eCD4-Ig induces changes in antibody binding to HIV envelope glycoprotein.
(A-G) HEK293T cells were transfected to express the BG505 Env with a deletion in its cytoplasmic tail to increase expression on the cell surface. Cells were pre-incubated with varying concentrations of eCD4-Ig with mouse Fc domains (eCD4-mIg), as indicated. Cells were washed and then incubated with 0.4 μg/mL of the indicated antibodies, and analyzed by flow cytometry. Mean fluorescence intensity values (MFI) are normalized to the value of antibody binding in the absence of eCD4-Ig or CD4-Ig. Error bars represent range (n = 2). Data are representative of at least three independent experiments.