Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells
Fig 5
OMVs lacking O antigen are biased towards clathrin-mediated endocytosis, while OMVs with O antigen can efficiently access host cells via lipid rafts.
Hela cells were either left untreated (control, red), or pre-treated with 5 μg/ml papain (lilac), 1 μg/ml chlorpromazine (pink), 5 mM methyl-β-cyclodextrin (light green) or 1 μg/ml filipin (turquoise), and exposed to ClyA-Bla OMVs isolated from EHEC (A), EAEC (B) or K12 (C) with or without O antigen at an MOI of 1000 for 3 hours. Total FRET changes after 3 hrs were determined from data in S6 Fig and data shown are means ± stdev (n = 3). Significance compared to the control group was determined using ANOVA, with a Brown Forsythe test to determine equal variance. (***) indicates p≤0.001, (**) p≤0.01, (*) p≤0.05, (ns) not significant.