Stably expressed APOBEC3H forms a barrier for cross-species transmission of simian immunodeficiency virus of chimpanzee to humans
Fig 7
Similar anti-SIVcpz activity of cpzA3H haplotypes.
(a) Four single nucleotide polymorphisms (SNPs) of cpzA3H were observed in 61 chimpanzees, see also Table 1. These four SNPs including reference cpzA3H were named haplotype I (hapI) to haplotype V (hapV). CDD, cytidine deaminase domain. (b) The polygenetic relationship of primate A3H proteins. 500 bootstrap replications were performed during calculation. The number on each node indicates the bootstrap support. (c) cpzA3H expression plasmids for different haplotypes were transfected into 293T cells. After two days, the expression of cpzA3H was detected by using anti-HA antibody, respectively. Tubulin served as a loading control. (d) SIVcpzPttMB897WT or SIVcpzPttMB897Δvif reporter constructs were co-transfected with expression plasmids for cpzA3H haplotypes into 293T cells, pcDNA3.1(+) empty vector was used as control (vector). Two days post-transfection, normalized amounts of virus were used to infect 293T cells. The nanoluciferase (relative light units-RLU) was measured 2 days post-infection. (e) The expression plasmids of cpzA3H haplotypes were co-transfected with expression plasmids for Vifs (from different SIVcpz strains, as indicated) into 293T cells. The presence of A3H and Vif were detected by using anti-HA and anti-Vif antibodies, respectively. Tubulin served as a loading control.