Stably expressed APOBEC3H forms a barrier for cross-species transmission of simian immunodeficiency virus of chimpanzee to humans
Fig 2
Chimpanzee APOBEC3s (cpzA3s) inhibit SIVs.
(a, b) SIVmac or SIVagm wild type or delta vif reporter viruses were produced in 293T cells in the presence of cpzA3s. pcDNA3.1(+) was used as control (vector) for cpzA3s. Two days post-transfection, normalized amounts of viruses were used to infect 293T cells, firefly luciferase (relative light units-RLU) was measured two days post-infection. (c, d) SIVcpzPttMB897 or SIVcpzPtsTAN1 wild type or delta vif reporter viruses were produced in 293T cells in the presence of cpzA3s. pcDNA3.1(+) was used as control (vector) for cpzA3s. Two days post-transfection, normalized amounts of viruses were used to infect 293T cells. Two days post-infection, 293T cells were carefully washed once with PBS, and nanoluciferase (relative light units-RLU) was measured, relative infectivity was shown. Values are means plus standard deviations (error bars) of a representative experiment performed in triplicate. Asterisks represent statistically significant differences: P value < 0.001 extremely significant (***), 0.001 to 0.01 very significant (**), 0.01 to 0.05 significant (*), >0.05 not significant (ns).