The yeast form of the fungus Candida albicans promotes persistence in the gut of gnotobiotic mice
Fig 5
RNA-seq mapping of the transcriptional regulatory network controlled by the identified regulators ZCF8, ZFU2 and TRY4.
(A) Plots show the distribution of all analyzed transcripts (∼6100 genes; each dot represents one transcript) when comparing the indicated deletion mutant to the wild-type reference strain. Plotted is the log2 fold change in expression (Y-axis) as a function of the mean of the normalized read counts (X-axis) for each transcript. Colored triangles correspond to transcripts whose expression is activated (i.e. positive regulation) (red) or repressed (i.e. negative regulation) (blue) by each regulator at Padj < 0.01. (B) Network view of the full set of genes whose expression is influenced by the identified C. albicans transcription regulators. The nodes of the network (in shades of green) are transcription regulators identified in our genetic screen. Large circles are composed of small colored ellipses. Each one of the latter represents an individual target gene. Positive (i.e. activation) and negative (i.e. repression) regulation is indicated by red and blue colors, respectively. The two black small ellipses depict the two genes that show discordant regulation (i.e. they are positively controlled by one regulator and negatively regulated by another). The shown network is completely based on RNA-seq data generated in this study.