Mycobacterium tuberculosis infection modulates adipose tissue biology
Fig 5
Genes associated with T cells, cytokines and chemokines are differentially upregulated in perigonadal and subcoutaneous fat post aerosol-infection with Mtb.
(A) Expression of Cd8a, Cd3d, Cd4, Ifng, Il4, Ccl5, Ccr7, Cxcl9, Cxcr3 (left panel) and Tbx21, Rorc and Gata3 (right panel) in perigonadal fat, lung and subcutaneous (SC) fat, as measured with quantitative PCR at day 28 post infection. (B) Expression of genes as in (A) in AD or SVF fractions of perigonadal fat at day 28 post infection. Gene expression is relative to the lower values detected and each group is compared to their own uninfected controls. Data are representative of two to three independent experiments (means); *p<0.05 and **p<0.01 (Student’s t-test). Abbreviations: AD, adipose fraction; SC, subcutaneous; SVF, stromal vascular fraction.