Mycobacterium tuberculosis infection modulates adipose tissue biology
Fig 2
Mtb expresses stress-related genes in perigonadal fat at different time points post aerosol-infection of mice.
(A) log10 Mtb CFUs in spleen, lung and perigonadal fat at different time points post aerosol-infection (200 CFUs). Data are representative of four independent experiments (medians). (B) log10 Mtb CFUs in spleen, lung and perigonadal fat at day 28 post aerosol-infection with 50 or 200 CFUs. Data are representative of two independent experiments (median); ** p<0.01 (Mann–Whitney test). (C) log10 Mtb CFUs in spleen, lung and AD and SVF fractions of perigonadal fat at different time points after aerosol infection. Data representative of two independent experiments (medians). (D) Expression of Mtb genes dosR, hspX and lat in the AD and SVF fractions of perigonadal fat at day 28 post aerosol-infection. Results of four independent experiments pooled (medians); *p<0.05 (Mann–Whitney test). (E) PCR specific for Mtb (IS 6110) in perigonadal fat at day 14 post aerosol-infection. Each lane represents a different mouse. Data representative of two independent experiments. (F) Immunofluorescence staining of perigonadal fat of mice infected i.v. with Mtb-GFP at 48 h after infection (arrows, green). Blue: DNA-intercalating dye Draq5. Data representative of two independent experiments. (G) log10 Mtb CFUs in spleen, lung, perigonadal (P) and subcutaneous (SC) fat from uninfected mice that received a subcutaneous injection of pergonadal fat from mice previously infected with 5x106 CFUs of Mtb. Organs were collected 14 days after the transfer of perigonadal fat. Data representative of two independent experiments (medians). Abbreviations: AD, adipose fraction; P, perigonadal; SC, subcutaneous; SVF, stromal vascular fraction.