The 5'-poly(A) leader of poxvirus mRNA confers a translational advantage that can be achieved in cells with impaired cap-dependent translation
Fig 2
The optimal length of 5’-poly(A) leader in conferring translational advantage is 12 residues.
(A) The Fluc reporter mRNAs with different 5’-poly(A)-leaders lengths were transfected into uninfected and VACV-infected HeLa cells, together with an Rluc mRNA. Luciferase activities were measured at 5 h post transfection. (B) Fluc reporter mRNA containing the A2L mRNA 5’-UTR with (w) or without (w/o) a poly(A) leader, was transfected into uninfected and VACV-infected HeLa cells, together with an Rluc mRNA. Luciferase activities were measured at 5 h post transfection. The Rluc-normalized Fluc activity was normalized as 1 in uninfected cells. (C) Fluc reporter mRNA containing RNF165 5’-UTR with (w) or without (w/o) an A-tract addition at the 5’-end was transfected into uninfected and VACV-infected HeLa cells together with an Rluc mRNA. Luciferase activities were measured at 5 h post transfection. Error bars represent standard deviation (SD) of at least three experiments. P- values were determined using the Student’s t-test; *P value < 0.05.