IL-10-mediated signals act as a switch for lymphoproliferation in Human T-cell leukemia virus type-1 infection by activating the STAT3 and IRF4 pathways
Fig 2
IL-10 enhanced expression of Ki67 and survivin in HAM/TSP-derived ILTs.
ILTs were cultured with or without IL-10 for 7–11 days, and subjected to flow cytometry (A, B) and immunoblot assays (C, D). A. Permeabilized cells were stained with anti-Ki67 (solid line) or control (closed histogram) antibodies. Numbers indicate MFI of Ki-67 expression. B. Cells were stained with Annexin V, and the proportion of apoptotic cells (%) was indicated as the mean and SD of 2–3 independent experiments. The representative flow cytometry data are shown in S2 Fig. C. ILTs cultured with or without IL-10 were treated with MG132 (10 μM) for 3 h, then subjected to immunoblot assays probed with antibodies to caspase-3, cleaved caspase-3, and β-actin. Approximate sizes (kDa) of caspases are indicated. The results of a similar experiment without MG132-treatment is shown in S3 Fig. D. ILTs cultured with or without IL-10 were subjected to immunoblot assays to detect survivin. The number under each band represents the relative value of survivin expression normalized against β-actin. Similar results were obtained in two independent experiments.