Cross-seeding of prions by aggregated α-synuclein leads to transmissible spongiform encephalopathy
Fig 4
Histopathological analysis of brains from the 2nd passage of PrPres produced in dgPMCAb reactions seeded with fibrillar WT α-synuclein.
Representative images of caudate putamen (a) and cerebellum (b) stained with hematoxylin and eosin, hippocampus stained with anti-PrP 3F4 (c), anti-GFAP (d) or anti-Iba1 antibody (e), or subventricular zones stained with anti-PrP 3F4 (f), anti-GFAP (g) or anti-Iba1 antibody (h). Subventricular deposits are indicated by arrows and the ventricular surface of ependymal cells is indicated by an arrowhead. S-O, stratum oriens; S-R, stratum radiatum; S-L, stratum lacunosum-moleculare; d, dentate gyrus, LV, lateral ventricle. Scale bars: in a, b, f, g, h = 100 μm, c, d, e = 500 μm.