Cross-seeding of prions by aggregated α-synuclein leads to transmissible spongiform encephalopathy
Fig 3
Histopathological analysis of brains from the 1st passage of dgPMCAb-derived PrPres.
Representative images of the frontal cortex (a, b, c, d) and hippocampus (e, f, g, h) of animals inoculated with dgPMCAb products seeded with fibrillar WT α-synuclein (a,b,e,f) or dgPMCAb products seeded with lysates of HeLa cells expressing A30P α-synuclein (c,d,g,h). Note the lack of spongiform change in the sections stained with hematoxylin and eosin (a, c, e, g) and patchy reactive astrogliosis (immunostaining for GFAP are in the insets in panels a and c). Immunostaining for PrP using SAF-84 (b, d, f, h) revealed diffuse/synaptic and granular deposits. Scale bar in a = 50 μm.