Structural basis of Zn(II) induced metal detoxification and antibiotic resistance by histidine kinase CzcS in Pseudomonas aeruginosa
Fig 5
Proline substitution of residues along the H1 and H1’ α-helices.
(a) The schematic diagram showing the locations of the residues involved in the proline substitutional experiments. The positions of Gln52, Leu50, Leu48, Asn45, Arg43, and Arg41 on the H1 and H1’ α-helices are labelled with circles in different colors. (b) Metal and antibiotic tolerance plate assay of single proline substitutions. Wild type P. aeruginosa and its derivative strains are examined on the LB plates that contain Zn(II) and MEPM antibiotic as follows: wild type P. aeruginosa with the empty pAK1900 plasmid as the control (WT PAO1 pAK1900), czcS deficient P. aeruginosa with empty pAK1900 (PAO1△czcS pAK1900), czcS-deficient P. aeruginosa with wild type czcS encoded on pAK1900 (PAO1△czcS pCSAK), czcS-deficient P. aeruginosa complemented with czcS mutants in pAK1900 (PAO1△czcS pCSAK Q52P, PAO1△czcS pCSAK L50P, PAO1△czcS pCSAK L48P, PAO1△czcS pCSAK N45P, PAO1△czcS pCSAK R43P, and PAO1△czcS pCSAK R41P).