Cucumber mosaic virus coat protein modulates the accumulation of 2b protein and antiviral silencing that causes symptom recovery in planta
Fig 7
CMV CP is associated with RDR6/SGS3 complex.
(A) Interactions detection of SGS3 with CPWT or CPRA in N. benthamiana leaves epidermal cells using bimolecular fluorescence complementation (BiFC). Leaves were infiltrated with pairs of Agrobacterium strains harboring tested proteins tagged with the different halves of YFP. Rubisco protein (Rub) was negative control. Images were taken at 2 dpi by confocal scanning laser microscopy. Bars represent 20 μm. (B) Co-immunoprecipitation (Co-IP) assays showing associations of SGS3 with CPs in vivo. N. benthamiana leaves coexpressing Flag-CPWT and Flag-CPRA proteins in combination with the GFP-SGS3 protein were homogenized and incubated with anti-Flag M2 antibodies for co-IP assays. GFP was included as a negative control. Anti-GFP and anti-Flag antibodies were used to detect accumulations of GFP-SGS3/GFP and CP, respectively. The positions of GFP-SGS3 and GFP are indicated by black and white triangles, respectively.