Cucumber mosaic virus coat protein modulates the accumulation of 2b protein and antiviral silencing that causes symptom recovery in planta
Fig 6
RNA binding and translation inhibition by the CPWT and CPRA proteins.
(A) RNA-binding abilities of the GST-CPWT and GST-CPRA proteins as assessed by digoxigenin-labeled CMV RNA4 or Luciferase (Luc) mRNA (See Materials and methods for details). GST served as a negative control. Coomassie brilliant blue (CBB) staining was used as the protein loading control. (B) Translation inhibition of Luciferase mRNA by CPWT and CPRA in vitro. The Luc mRNA was transcribed and incubated with different concentrations of the GST, GST-CPRA, or GST-CPWT in wheat germ extract at 25°C for two hours. Then, the activity of translated Luciferase in vitro was measured with a luminometer. Luciferase activities from mRNA without GST, GST-CPRA, or GST-CPWT was set as 100%. Error bars represent the standard error of the mean. Data points are the mean value of three independent experiments. *P-value < 0.05; ** P-value < 0.01; *** P-value < 0.001.