Cucumber mosaic virus coat protein modulates the accumulation of 2b protein and antiviral silencing that causes symptom recovery in planta
Fig 2
Distribution of CMVRA or CMVWT in the apical meristems of infected plants.
In situ hybridization of longitudinal sections of shoot apices in N. benthamiana plants inoculated with CMVRA and CMVWT at 7-, 14-, and 21- dpi. Dark areas indicate the presence of viral RNA revealed by digoxigenin-labeled riboprobe corresponding to the CMV CP. CMV signals were not observed in the shoot meristem from uninfected samples (left panel). Only a low signal density was present in CMVWT infected meristems at 7 dpi and these disappeared from the meristem by 14 dpi (middle panels). In contrast, CMVRA infection contained abundant signal densities beneath the meristems at 7 dpi, and indicated partially invaded meristems by 14 dpi, and completely invaded meristems at 21 dpi (right panels). Bars = 100 μm.