CD4 is expressed on a heterogeneous subset of hematopoietic progenitors, which persistently harbor CXCR4 and CCR5-tropic HIV proviral genomes in vivo
Fig 6
HSPCs with greater CD4 expression are preferentially infected by HIV Envs.
A. Representative flow cytometry plots and gating strategy for cord blood-derived CD133-sorted cells infected with virus containing the indicated envelope protein and harvested 3 days post-infection. Gating for CD4 was determined by the inclusion of 1% of cells stained with an isotype control antibody (gray). For GFP plots, numbers indicate the percentage of GFP+ events. B. Summary graphs depicting the ratio of infected cells in CD4high versus CD4low/- subsets of cord blood-derived HSPCs infected and analyzed as in part A. For SV16, two replicates had 0.0% infection in the CD4low/- gate leading to an undefined ratio, so 30.0 was used as a conservative estimate of the ratio. Data from 11 uninfected cord blood experiments. Mean ± standard deviation is indicated; n≥3 for each envelope. Results were compared to infection by VSV-G pseudotyped viruses and p values were determined using 2-tailed Student’s t-test (***p<0.001, ****p<0.0001). C. Data from B but compiled by tropism. Mean ± standard deviation is indicated; one-way ANOVA, p<0.0001, with Tukey’s Multiple Comparisons Test indicated (***p<0.001 and ****p<0.0001).