HIV-1 infection depletes human CD34+CD38- hematopoietic progenitor cells via pDC-dependent mechanisms
Fig 5
Depletion of pDCs during chronic HIV-1 infection rescues early CD34+CD38- HPCs in humanized mice.
(A) Representative dot plots showing the recovery of early CD34+CD38- HPCs in BM of chronic HIV-1-infected humanized mice with pDC depletion. Numbers indicate percentages of early CD34+CD38- HPCs and intermediate CD34+CD38+ HPCs in various groups of mice. (B and C) Summary data of percentages (B) and absolute cell numbers (C) of early CD34+CD38- HPCs and intermediate CD34+CD38+ HPCs from BM in mock (n = 11), HIV-1-infected (n = 12) and HIV-1-infected humanized mice with pDC depletion (n = 13). Each dot represents one mouse. *P < 0.05 and ***P < 0.001 (two-tailed unpaired Student’s t-test). (D) Summary data of CFU that developed from CD34+ HPCs of mock-infected mice (n = 4), HIV-1-infected mice (n = 5) and HIV-1-infected mice with pDC depletion (n = 4). *P < 0.05 (two-tailed unpaired Student’s t-test). Error bars, s.e.m