HIV-1 infection depletes human CD34+CD38- hematopoietic progenitor cells via pDC-dependent mechanisms
Fig 2
Chronic HIV-1 infection preferentially depletes early CD34+CD38- HPCs in humanized mice in vivo.
(A) Representative histograms showing p24 expression on CD3+ T cells and CD34+ HPCs from BM of humanized mice with chronic HIV-1 infection. Numbers indicate percentages of p24-expressing CD3+ T cells and CD34+ HPCs. Data are representative of three independent experiments with mice reconstituted with two to three donors. (B) Representative dot plots showing chronic HIV-1 infection depleting early CD34+CD38- HPCs from BM of humanized mice. Numbers indicate percentages of early CD38-CD34+ and intermediate CD34+CD38+ HPCs. (C) Pooled data indicating mean percentages of early CD34+CD38- HPCs and intermediate CD34+CD38+ HPCs in BM of humanized mice with mock and HIV-1 infection shown in the stacked bar graph. P values are shown. (D-E) Pooled data indicating absolute cell numbers of early CD34+CD38- HPCs (D) and intermediate CD34+CD38+ HPCs (E) in BM of humanized mice with mock (n = 7) or chronic HIV-1 infection (n = 5). (F) Representative dot plots showing chronic HIV-1 infection depleting early CD34+CD38- HPCs from BM of HIV-1-infected patients compared to that of healthy control (HC) donors. Numbers indicate percentages of early CD38-CD34+ and intermediate CD34+CD38+ HPCs. (G-H) Pooled data indicating percentages of early CD34+CD38- HPCs (G) and intermediate CD34+CD38+ HPCs (H) in BM of HC donors (n = 6) and HIV-1-infected patients (n = 5). (D, E, G, H) Data are shown as the mean ± s.e.m. *P < 0.05 and ***P < 0.001 (two-tailed unpaired Student’s t-test).