LMP1-mediated glycolysis induces myeloid-derived suppressor cell expansion in nasopharyngeal carcinoma
Fig 5
NLRP3 expression was involved in the accumulation of NPC-LMP1-mediated MDSCs.
(A) Representative immunoblots of CNE2-LMP1 and TW03-LMP1 cells treated with siNC or siNLRP3 and stained with the indicated antibodies (n = 3). (B) Secretion of the cytokines IL-1β, IL-6 and GM-CSF from NPC-LMP1 cells following treatment with si-NLRP3, siControl (siNC) or VX-765 was determined via ELISA. (C) Blockade of NLRP3 decreased the differentiation of HLA-DR-CD11b+CD33+ MDSCs mediated by NPC-LMP1. Representative data from 5 independent experiments are shown. (D) Statistical analysis of the percentage of CD33+CD11b+HLA-DR-MDSCs mediated by CNE2-LMP1 or TW03-LMP1 cells following treatment with si-NLRP3, siNC or VX-765. Representative data from 3 independent experiments are shown.