Adaptation of Candida albicans to environmental pH induces cell wall remodelling and enhances innate immune recognition
Fig 10
Enhanced immune recognition of acidic adapted cells is mediated via Dectin-1.
a) Fc-Dectin-1 binding to wild type C. albicans cells grown to mid-log phase in YPD buffered to the appropriate pH, as quantified by FACS counting 10,000 events per repeat. Fold increased is relative to unbuffered YPD. b) C. albicans was grown in YPD at the appropriate pH to mid-log phase, co-incubated with fibroblasts for 1 h, fixed and the association index (number of fungal cells either attached and phagocytosed/100 macrophages) determined. c) Association of acidic adapted C. albicans cells to fibroblasts expressing Dectin-1 in the presence of glucan phosphate. d) J774.1A macrophages were pre-incubated with glucan phosphate and infected with C. albicans at an MOI of 5 and the phagocytosis index (number of fungal cells phagocytosed/100 macrophages) determined after 1 h. e) J774.1A macrophages were pre-incubated with glucan phosphate and infected with C. albicans at an MOI of 5 and the association index determined after 1 h. Data represent the mean ± SEM from three independent repeats (* p > 0.05. *** p > 0.001).