Adaptation of Candida albicans to environmental pH induces cell wall remodelling and enhances innate immune recognition
Fig 2
Adaptation to acidic environments promotes surface exposure of chitin.
a) Wild type cells (NYG152) were grown in YPD buffered at the appropriate pH, stained with CFW and fluorescence quantified by FACS. The fold increase in CFW staining was determined from the background subtracted MFI values from FACS analysis, and normalised to YPD grown cells. b) Fold increase in FITC fluorescence of FITC-WGA stained wild type cells (NYG152) grown at different pH, relative to growth in YPD as quantified by FACS analysis. All data represent the mean ± SEM from three independent experiments. c) Microscopy of WGA stained cells. Arrowheads indicate chitin exposure. Scale bar = 10 μm.