Mycobacterium tuberculosis subverts negative regulatory pathways in human macrophages to drive immunopathology
Fig 7
The PI3K and MNK pathways converge at the level of eIF4E phosphorylation.
A. Inhibition of eIF4F complex formation significantly suppresses MMP-1 secretion from Mtb-infected macrophages at 72h. B. Insulin, a PI3K pathway activator, increases macrophage eIF4E phosphorylation, while LY294002 abrogates phosphorylation. Mtb infection does not change basal eIF4E phosphorylation levels, but either MNK or PI3K/PD-1 inhibition suppresses eIF4E phosphorylation at 90 mins. A: representative data from an experiment performed in 2 donors in triplicate, B: performed on three occasions in different donors. Mean and standard deviation shown and p values are by Student’s t-test.