Mycobacterium tuberculosis subverts negative regulatory pathways in human macrophages to drive immunopathology
Fig 2
PI3K inhibition has global effects on MMP, cytokine and chemokine secretion by macrophages.
Luminex profiling of MMPs, cytokines, chemokines and growth factors demonstrates that Mtb infection significantly upregulates a wide range of mediators from macrophages at 72h. Secretion of the majority of mediators was further augmented when PI3K signalling was globally inhibited. Median values normalised to Mtb-infected samples of an experiment performed in triplicate are shown, and are representative of an experiment performed on two separate occasions.