Genetically-barcoded SIV facilitates enumeration of rebound variants and estimation of reactivation rates in nonhuman primates following interruption of suppressive antiretroviral therapy
Fig 5
Plasma viral loads, PBMC CA-vRNA and CA-vDNA in animals in study 2.
(A) 6 rhesus macaques, DEJX (red), DFGV (yellow), DEJW (blue), H090 (purple), DEPI (light green), and H105 (dark green) were infected with SIVmac239M and cART was initiated on day 4 post-infection. Pairs of macaques were removed from therapy on days 305, 374, and 482 post-infection. Viral RNA copies were measured in plasma collected up to 550 days. Bars over the viral load data indicate duration of therapy for each animal. (B-C) Cell-associated viral DNA (B) and RNA (C) was measured in PBMCs over the duration of cART therapy in animals infected with SIVmac239M. Measurements obtained are shown overlaying the detected plasma viral loads. Open symbols represent measurements that were below the limit of detection.