Gre factors-mediated control of hilD transcription is essential for the invasion of epithelial cells by Salmonella enterica serovar Typhimurium
Fig 5
Overexpression of HilD restores HilA expression and epithelial cell invasiveness in ΔgreAΔgreB strains.
(A) In lower panels, immunodetection of the HilA-FLAG protein in whole cell extracts from cultures of WT and ΔgreAΔgreB derivative strains carrying either pBAD18 or pBADHilD grown in LB at 37°C up to an OD600nm of 2.0, arabinose (0.02%) was added in all cultures. The upper panels are sections of Coomassie stained gels as loading controls. (B) Invasion assays using WT, ΔmotA and ΔgreAΔgreB strains carrying the indicated plasmids. Bacterial cultures were grown as in Fig 1. A bar shows the arithmetic mean of experimental results and the error bar indicates the standard deviation.