The redefinition of Helicobacter pylori lipopolysaccharide O-antigen and core-oligosaccharide domains
Fig 2
MS analysis of H. pylori Wild-type G27 LPS.
Wild-type G27 LPS samples were subjected to (A): methanolysis; (B): mild HF hydrolysis, and (C): mild periodate oxidation, respectively. MALDI-TOF MS spectra were recorded after permethylation. The MS peaks corresponding to sodiated glycans are coloured red, and annotated with m/z values and glycan structures. Note that for the spectrum after mild HF hydrolysis, the most intense isotopic peaks are annotated. Other blank signals are mainly due to (A): an addition of a sodium atom; and (C): incomplete reduction. The MS data indicate the fundamental architecture of wild-type G27 LPS is the same as strain 26695, containing LacNAc, heptan, glucan, Trio, the phosphorylated Glc-Gal-tri-Hep-KDO structure and lipid A.