Exosome secretion affects social motility in Trypanosoma brucei
Fig 5
(A) Immunogold TEM analysis demonstrating the location ZC3H41 in ILVs within MVBs. Cells carrying the SmD1 silencing construct, and the YFP-VPS36 construct were used. (a, b) un-induced (-Tet) or (c-g) silenced for 40 hrs. (+Tet) were used to prepare Cryo-EM sections, and were subjected to immunogold analysis. Imaging was performed using antibody to ZC3H41. (B) as in (A), but using anti-GFP antibody to detect the VPS36. The scale bars are indicated.