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Structure of a pentameric virion-associated fiber with a potential role in Orsay virus entry to host cells

Fig 5

CP-δ forms particle associated fibers.

(a) EM images of Orsay VLPs containing CP-δ. Protruding fibers are highlighted by orange stars. Scale bar, 500 Å. (b) Length measurements of particle-associated fibers in comparison with the full-length δ fibers. (c) SDS-PAGE of the Orsay VLP. Western blot was performed using an anti-His antibody. (d) An Orsay capsid model with CP-δ fibers situated at the five-folds. On five-fold vertices, the CP portion and the δ portion of the CP-δ are shown in light green and orange respectively. The rest of the CP molecules in the capsid are shown in dark green. The Orsay, ~350-Å in diameter, and the CP-δ fiber, ~400-Å in length, are approximately drawn to scale.

Fig 5

doi: https://doi.org/10.1371/journal.ppat.1006231.g005