Translocation of the papillomavirus L2/vDNA complex across the limiting membrane requires the onset of mitosis
Fig 2
L2 translocation requires endosome acidification and cyclophilin activity.
(A) Representative translocation blot from asynchronous HaCaT-GFP-BAP cells infected with 400 ng L1/ml of L2-BirA pseudovirions for indicated times. (B) Infection levels in HaCaT GFP-BAP cells infected with 300 ng L1/ml of L2-BirA or L2-HA PsV for the indicated times. Infection levels are expressed relative to the 16 hour time point, which is set at 100%. (C) Infection and translocation in HaCaT GFP-BAP cells infected with L2-BirA PsV in the presence of vehicle, the cyclophilin inhibitor cyclosporin A (CsA), or the endosomal acidification inhibitors bafilomycin A (BafA) and ammonium chloride (NH4Cl). Infection levels are expressed relative to vehicle-treated cells, which are set at 100%. All infection values represent mean percent infection (±SEM, n = 2), normalized to GAPDH.