Characterization of Early-Phase Neutrophil Extracellular Traps in Urinary Tract Infections
Fig 2
Evidence of neutrophil death, disintegration and multi-cellular aggregation in AUP samples.
Urine sediment samples were centrifuged at 1,300 x g for 5 min and gently resuspended in PBS. Aliquots were stained with Trypan Blue (1:10 dilution) and subjected to phase-contrast microscopy (40-fold magnification). White arrows denote bright viable cells; yellow arrows denote dead cells, some with a flattened morphology and larger and some with visible nuclei stained blue; red arrows denote ruptured cells with membrane borders no longer clearly visible, some with dark patches of apparently released chromatin (especially in AUP samples #151 and #157).