TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP)
Fig 2
Secondary screen confirms the ZAP-dependent and -independent antiviral effects of hits.
A customized library of individual siRNAs (Ambion) targeting 102 hits from the primary screen was tested in triplicate in two different cell lines. Distribution of average Z scores for each of the 3 siRNAs targeting a candidate gene in the secondary screen is shown here. The screen was performed with (A) 6.25 nM or (B) 25 nM individual siRNAs in 293 cells induced to express ZAPS (T-REx-hZAP), and with (C) 6.25 nM or (D) 25 nM individual siRNAs in 293 cells induced to express the rZAPC88R dominant negative mutant (T-REx-rZAPC88R). Each dot represents the average Z score of an individual siRNA tested in triplicate. Silenced genes with an average Z score of >3 for at least 2 out of 3 siRNAs are identified and the siRNAs are labeled in color. (C and D) The average Z scores of TRIM25 and ZC3HAV1 are also plotted to indicate that TRIM25 does not have ZAP-independent antiviral effects.