Gene Expression and Silencing Studies in Phytophthora infestans Reveal Infection-Specific Nutrient Transporters and a Role for the Nitrate Reductase Pathway in Plant Pathogenesis
Fig 9
Expression of P. infestans NR in artificial media cultures under different conditions.
A. Relative levels of NR mRNA in wild-type P. infestans grown on rye-sucrose agar exposed to constant dark, constant light, or a 12 hour light/dark cycle. In this and the other panels, error bars represent biological replicates and unless specified otherwise cultures were maintained at high humidity (>95% RH). B. NR mRNA in 2 and 5-day cultures of wild-type P. infestans grown on rye-sucrose or pea media. Both 5-day cultures were sporulating. C. NR mRNA in 5-day cultures of wild-type P. infestans grown on rye-sucrose agar at high or low humidity (>95% and ~30%, respectively). D. NR mRNA in rye-sucrose agar cultures of wild type P. infestans and two independent Cdc14-silenced transformants; the latter do not sporulate. E. NR RNA in wild type P. infestans grown for 3 days on rye-sucrose agar or rye-sucrose broth; neither culture was sporulating at the time of harvest.