Pyrimidine Salvage Enzymes Are Essential for De Novo Biosynthesis of Deoxypyrimidine Nucleotides in Trypanosoma brucei
Fig 2
TK is essential for in vitro growth and infectivity in mice.
A. Growth analysis of TK c-null cells and wild-type SM cells ±Tet. Expression of ectopic FLAG-tagged TbTK is under Tet control, thus removal of Tet leads to loss of TbTK expression. Cells were grown in HMI-19 medium supplemented with either normal serum (NS) or dialyzed serum (DS). Cell growth was monitored for the indicated days. Error bars represent standard deviation (SD) for triplicate biological replicates. Inset shows western blot analysis of FLAG-tagged TbTK expression ±Tet for 24h. TbBiP was detected as a loading control. B. qPCR analysis comparing mRNA expression levels of TbTK to the TERT control ±Tet for 24 h. Error bars represent standard error of the mean (SEM) for triplicate data. C. Survival analysis of wild-type SM and TK c-null infected mice (±Dox) 1–30 days post infection for three mice per group.