The KSHV K1 Protein Modulates AMPK Function to Enhance Cell Survival
Fig 5
The K1 N-terminus is important for association with AMPKγ1.
(A) HEK-293 cells stably expressing empty vector (EV) or FLAG-K1 (K1) were transfected with V5-AMPKγ1 (AMPKγ1). AMPKγ1 was immunoprecipitated, and blots were probed for AMPKγ1, endogenous AMPKα1, endogenous AMPKβ1 and K1. (B) Endogenous AMPKβ1 was immunoprecipitated from EV or K1 expressing HEK-293 cells. The blots were probed for K1 or AMPKβ1. (C) HEK-293 cells were transiently transfected with AMPKγ1 and with one of the following: K1WT, K1ΔCT or K1ΔNT. An equivalent amount of EV (pcDNA3) was also transfected along with K1WT. We immunoprecipitated the various K1 domain mutants, and immunoblotted for AMPKγ1 or K1.